Our cryopreservation program has been utilising the vitrification method since 2007.
The astonishing survival rate of this method is a powerful tool for our lab to increase the cumulative success rate from one stimulated cycle either by fresh or frozen embryo transfer. Vitrification is characterised as the second most ‘important invention’ in IVF world after Intracytoplasmic Sperm Injection (ICSI) due to its importance in the routine work of an IVF laboratory. It is proven that in stimulated cycles endometrial receptivity is impaired due to stimulation drugs. Thus a subsequent frozen embryo transfer could bypass this adverse effect and result in a successful pregnancy with similar or even higher pregnancy rate than fresh cycles. We are strong supporters of this idea but more studies need to be conducted before we turn to a freezing all embryos strategy. Vitrification of embryos in Pedieos IVF Center is applied for:
- Storing supernumerary embryos from an IVF cycle
- Patients with a risk of Ovarian Hyperstimulation Syndrome. Transfer will take place in a future non stimulated cycle with success rate similar to a fresh cycle
- Poor responder cycles (>39 or POF, ↓AMH). Good quality embryos from several natural or minimal stimulation cycles are vitrified. Normally we will have 1 or 2 good quality embryos maximum collected from each cycle. When enough good quality embryos are collected and vitrified, we will schedule the frozen embryo transfer maximizing the chance of pregnancy for these patients.
Frozen embryo transfer could be done either on cleavage stage embryos (D2 or Day 3) or Day 5 (blastocyst transfer). We perform Laser Assisted Hatching to all of our warmed embryos before transfer free of charge. Our stats show that using vitrified embryos to a subsequent non stimulated cycle has similar or even better pregnancy rates than a fresh transfer.